The Sorption of Influenza Virus by Chicken
نویسنده
چکیده
The nature of the forces of attraction between cells and viruses is of obvious interest; and although erythrocytes probably are not the cells of primary importance in influenza virus infections, the attractive forces between them and influenza virus provide an experimental approach to the problem. Previous reports presented data which indicate that the effect of influenza virus upon erythrocytes is influenced to a considerable extent by forces which appear to be of a physical-chemical nature. Failure of the WS strain of influenza virus to agglutinate sheep erythroeytes was shown to result from several sets of forces, some of which tended to agglutinate sheep erythrocytes and others of which tended to inhibit agglutination; over a narrow pH range the conditions could be controlled so that agglutination was snfl~ciently satisfactory to permit use of sheep erythrocytes for agglutination-inhibition tests (1). Furthermore, the WS strain of virus, which was in the phase of variation in which it agglutinated guinea pig but not chicken erythrocytes under usual test conditions, was shown to agglutinate erythrocytes of both species when the system was suitably buffered (2). Those data are of interest because i n the former instance they showed that failure of the virus to agglutinate sheep erythrocytes was not due to lack of "receptors," as has been supposed (3), and in the latter instance they showed that the failure of the "O"-like phase of virus to agglutinate chicken erythrocytes was not due to the lack of aifinity of the variant for that species of ceils. The present paper includes additional evidence of the influence of physicalchemical forces upon reactions between influenza virus and erythrocytes. The data indicate that the forces of attraction between virus and chicken erythrocytes are governed by the laws of mass action or, perhaps, by the laws of simple adsorption. Virus is sorbed I by erythrocytes in a manner which effects a pro-
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تاریخ انتشار 2003